JLB Targeted Science Issue: Neutrophil Flow Cytometry Research and Methodologies
We are excited to announce a special call for manuscripts focusing on the classical and new innovative methodologies employed in the study of neutrophils using flow cytometry.
Scope of the Targeted Science Issue:
This special issue aims to bring together cutting-edge Neutrophil Flow Cytometry Research and Methodologies. We are interested in manuscripts covering a broad range of topics, including but not limited to:
Methods Topics:
- Basic multicolor flow cytometry: neutrophil isolation from tissues, preserving viability and activation state; phenotypic characterization-gating strategies; marker validation, standards, and controls; intracellular staining; experimental design considerations for neutrophil FACS
- Marker selection and panel design for interrogating neutrophil heterogeneity and plasticity: development/maturation, neutrophil "subsets," assessing neutrophil purity; markers across species; evolution of neutrophils/neutrophil-like cells
- Application of flow cytometry in studying neutrophil activation/function: priming, phagocytosis, NETosis, ROS, intracellular cytokines, membrane potential/kinetics
- Flow cytometry methods to integrate sorting with downstream applications like RNA-seq or other omics approaches
Methods Analysis Topics:
- Unbiased, conceptual comparison of neutrophil heterogeneity and cell states derived from various methodologies, including flow cytometry
- Overview/comparison of applications of state-of-the-art flow cytometry methods to study neutrophils (e.g., CyTOF, imaging cytometry, omics cytometry)
- Method dependence of data interpretation, e.g., flow cytometry vs. immunofluorescence staining
- Interpreting high/multi-dimensional neutrophil data for the big data non-expert: explore and explain how multi-parametric flow cytometry can be integrated with other omics technologies to comprehensively understand neutrophil biology
- Flow cytometry data analysis using mathematical and bioinformatical tools
Submission Guidelines:
Important Dates:
- Submission deadline: May 31, 2024 (please communicate with our managing editors at jlbstaff@leukocytebiology if you need more time)
- Publication: all accepted manuscripts will be immediately published online; the complete issue is scheduled to be compiled in March 2025
Your expertise and contributions will significantly enhance the depth and breadth of this special issue. Please feel free to contact us if you have any questions or if you would like to discuss your potential submission further ([email protected]).
Thank you for considering JLB for disseminating your research, protocols, and knowledge. We look forward to receiving your manuscripts.
Deputy editor: Michael Schnoor
Guest Editors: Sven Brandau, Marco Cassatella, Sergio Catz, Liwu Li, Lai Guan Ng, and Silvia Uriarte
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